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alternative field electrophoresis  (Bio-Rad)


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    Structured Review

    Bio-Rad alternative field electrophoresis
    Alternative Field Electrophoresis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alternative+field+electrophoresis/Native+Sample+Buffer+for+Protein+Gels/10__4315_slash_jfp___22___167-58-75-82
    Average 95 stars, based on 129 article reviews
    alternative field electrophoresis - by Bioz Stars, 2026-10
    95/100 stars

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    Incubation:

    Article Title: Study of Virulence Genes, Antimicrobial Resistance, and Genetic Relatedness of Foodborne Salmonella Isolates from Tunisia
    Article Snippet: Bacterial cells were washed in TES buffer (10 mM Tris [pH 7.5], 10 mM EDTA [pH 8], 0.5% SD), before being suspended in 400 μL of TE buffer (1 M Tris-Cl [pH 8], 0.5 M EDTA [pH 8]) containing 10 mg/mL of lysozyme (New England Biolabs, Hitchin, UK). .. The cells were then incubated at 378C for 1 h. Lysed cells were introduced into agarose blocks and incubated 2 h at 558C with 20 mg/mL proteinase K. Enzymatic digestion was then realized on slices cut from the prepared blocks by using the endonuclease XbaI (New England Biolabs) at 258C for 4 h. Agarose plugs inserted into the agarose gel (Gibco BRL, Paisley, Scotland; 1.5% m/v in 0.53 TBE buffer) were subjected to a transverse alternative field electrophoresis (CHEF-DR R II apparatus, Bio-Rad, Hercules, CA) with the following parameters: 200 V, 2 s of initial switch time, 20 s of final switch time, and 18 to 24 h of migration at 148C. .. Each gel contained two λ PFGE ladders (λ DNA cI857 ind 1 Sam7, New England Biolabs).

    Agarose Gel Electrophoresis:

    Article Title: Study of Virulence Genes, Antimicrobial Resistance, and Genetic Relatedness of Foodborne Salmonella Isolates from Tunisia
    Article Snippet: Bacterial cells were washed in TES buffer (10 mM Tris [pH 7.5], 10 mM EDTA [pH 8], 0.5% SD), before being suspended in 400 μL of TE buffer (1 M Tris-Cl [pH 8], 0.5 M EDTA [pH 8]) containing 10 mg/mL of lysozyme (New England Biolabs, Hitchin, UK). .. The cells were then incubated at 378C for 1 h. Lysed cells were introduced into agarose blocks and incubated 2 h at 558C with 20 mg/mL proteinase K. Enzymatic digestion was then realized on slices cut from the prepared blocks by using the endonuclease XbaI (New England Biolabs) at 258C for 4 h. Agarose plugs inserted into the agarose gel (Gibco BRL, Paisley, Scotland; 1.5% m/v in 0.53 TBE buffer) were subjected to a transverse alternative field electrophoresis (CHEF-DR R II apparatus, Bio-Rad, Hercules, CA) with the following parameters: 200 V, 2 s of initial switch time, 20 s of final switch time, and 18 to 24 h of migration at 148C. .. Each gel contained two λ PFGE ladders (λ DNA cI857 ind 1 Sam7, New England Biolabs).

    Electrophoresis:

    Article Title: Study of Virulence Genes, Antimicrobial Resistance, and Genetic Relatedness of Foodborne Salmonella Isolates from Tunisia
    Article Snippet: Bacterial cells were washed in TES buffer (10 mM Tris [pH 7.5], 10 mM EDTA [pH 8], 0.5% SD), before being suspended in 400 μL of TE buffer (1 M Tris-Cl [pH 8], 0.5 M EDTA [pH 8]) containing 10 mg/mL of lysozyme (New England Biolabs, Hitchin, UK). .. The cells were then incubated at 378C for 1 h. Lysed cells were introduced into agarose blocks and incubated 2 h at 558C with 20 mg/mL proteinase K. Enzymatic digestion was then realized on slices cut from the prepared blocks by using the endonuclease XbaI (New England Biolabs) at 258C for 4 h. Agarose plugs inserted into the agarose gel (Gibco BRL, Paisley, Scotland; 1.5% m/v in 0.53 TBE buffer) were subjected to a transverse alternative field electrophoresis (CHEF-DR R II apparatus, Bio-Rad, Hercules, CA) with the following parameters: 200 V, 2 s of initial switch time, 20 s of final switch time, and 18 to 24 h of migration at 148C. .. Each gel contained two λ PFGE ladders (λ DNA cI857 ind 1 Sam7, New England Biolabs).

    Migration:

    Article Title: Study of Virulence Genes, Antimicrobial Resistance, and Genetic Relatedness of Foodborne Salmonella Isolates from Tunisia
    Article Snippet: Bacterial cells were washed in TES buffer (10 mM Tris [pH 7.5], 10 mM EDTA [pH 8], 0.5% SD), before being suspended in 400 μL of TE buffer (1 M Tris-Cl [pH 8], 0.5 M EDTA [pH 8]) containing 10 mg/mL of lysozyme (New England Biolabs, Hitchin, UK). .. The cells were then incubated at 378C for 1 h. Lysed cells were introduced into agarose blocks and incubated 2 h at 558C with 20 mg/mL proteinase K. Enzymatic digestion was then realized on slices cut from the prepared blocks by using the endonuclease XbaI (New England Biolabs) at 258C for 4 h. Agarose plugs inserted into the agarose gel (Gibco BRL, Paisley, Scotland; 1.5% m/v in 0.53 TBE buffer) were subjected to a transverse alternative field electrophoresis (CHEF-DR R II apparatus, Bio-Rad, Hercules, CA) with the following parameters: 200 V, 2 s of initial switch time, 20 s of final switch time, and 18 to 24 h of migration at 148C. .. Each gel contained two λ PFGE ladders (λ DNA cI857 ind 1 Sam7, New England Biolabs).



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